![]() ![]() ![]() wearing of shoes, access to clean water and sanitation, hand washing after defecation, cleaning of foods, and avoidance of wastewater) is considered sufficient to prevent transmission of these infections, studies systematically implementing such strategies (e.g. Although avoidance of such contacts (e.g. Parasite transmission is considered to occur through contacts with contaminated soil, food and drinking water. Transmission of intestinal parasites is generally by ingestion or skin contact with infectious parasite stages. Giardia lamblia) and soil-transmitted helminths (e.g. Common intestinal parasitic infections of childhood include protozoans (e.g. ![]() Intestinal parasite infections are estimated to infect more than a billion humans worldwide and are a major cause of morbidity, especially in children living in conditions of poverty in low- and middle-income countries. Our results indicate widespread contamination of households with parasite DNA and raise the possibility that beds, under conditions of overcrowding in a humid tropical setting, may be a source of transmission. To our knowledge, this is the first use of qPCR on environmental samples to detect a wide range of enteric pathogen DNA. ![]() lumbricoides DNA in bed (adjusted OR: 10.0, 95% CI: 2.0–50.1) but not floor dust (adjusted OR: 3.6, 95% CI: 0.3–37.9) was significantly associated with active infections in children. Bed dust was more frequently positive than floor samples for all parasites detected. Dust positivity rates for individual pathogens were: S. hominis (79% of household samples) indicating a high level of environmental fecal contamination. Of the 38 households sampled, 37 had positive dust for at least one parasite and up to 8 parasites were detected in single samples. cati, Giardia lamblia, Blastocystis hominis, Cryptosporidium spp., and Entamoeba histolytica) by quantitative PCR (qPCR). Dust was examined for presence of DNA specific for 11 enteric parasites ( Ascaris lumbricoides, Trichuris trichiura, Ancylostoma duodenale, Necator americanus, Strongyloides stercoralis, Toxocara canis and T. We collected bed and living room dust samples from households of children participating in a clinical trial of anthelmintic treatment in rural coastal Ecuador. Enteric parasites are transmitted in households but few studies have sampled inside households for parasites and none have used sensitive molecular methods. ![]()
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